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Figure 1 | Journal of Translational Medicine

Figure 1

From: Regulatory T cell frequency in patients with melanoma with different disease stage and course, and modulating effects of high-dose interferon-α 2b treatment

Figure 1

Flow cytometry: percentage of CD4+CD25+HFFoxp3+ cells in interferon-α 2b-treated patients with melanoma. Flow cytometric gating strategy to identify Treg cells. (A) Dot plot of forward scatter (FSC) versus side scatter (SSC) for all events: all peripheral blood cell populations are shown in red; the population of lymphocytes is shown in green. (B) Lymphocytes (green) were analyzed on the basis of surface markers CD4 and CD25; the P3 gate identifies the percentage of CD4+CD25+HF cells. (C) The P4 gate identifies the percentage of Foxp3+CD4+CD25+HF cells; this represents the region used to calculate the final percentage of Treg cells in CD4+ lymphocytes.

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