Figure 5
Comparison of tumor ablation and systemic immune response induced by two different scan strategies. (A) Thermal lesions produced by dense- and sparse-scan strategies in MC-38 tumors. (B-C) The suppressive effects of different scan strategies on the growth of treated primary tumors. (D-E) The retarding effects on the growth of distant re-challenged tumors. (F-G) Tumor-specific IFN-γ-secreting cells detected in the splenocytes of HIFU-treated mice. C57BL/6 mice were inoculated s.c. on right hind leg with 5 × 105 MC-38 or B16 tumor cells and treated with different HIFU on day 9 of tumor inoculation. Mice were challenged with 1 × 106 MC-38 or B16 tumor cells by s.c. inoculation on the left hind leg one day after HIFU treatment. Both primary and challenged tumor growth was monitored daily. Tumor-specific IFN-γ-secreting cells were detected in splenocytes by ELISPOTS assays. Results were expressed as mean ± SD for each group (n = 8 per group). *P < 0.05; **P < 0.001 versus non-treatment control by Student's t test. This experiment is representative of three experiments with consistent results.