Figure 4

DC maturation stimulated by HIFU-treated tumor cells. (A) Temperature profiles produced by 55°C-HIFU and 80°C-HIFU. (B) Immature DCs were incubated for 2 days in the presence of CpG-ODN, normal B16 cells, 55°C-HIFU and 80°C-HIFU treated B16 cells. Levels of IL-12 p70 and IL-10 in the culture supernatants were measured by ELISA. (C) Expression of CD80 and CD86 on the surface of DC (thick line) was assayed by Flow cytometry. Solid thin line represents the expression of these markers on surface of non-stimulated DC. Representative data out of three separate experiments are shown. (D) The expression levels of CD80 and CD86 on DCs were presented as mean fluorescence intensity. Results in panels B and D are expressed as means ± SD out of three independent experiments. * p < 0.05 compared with 'DC Alone', ^# p < 0.05 compared with 'DC+normal B16', ^! p < 0.05 compared with 'DC+80°C-HIFU' by Student's t test.