Figure 4

Effects of ezrin on cell motility in vitro. BioCoat Chambers were used to detect cell migration and representative fields were photographed. Black-arrows indicate the 8-µm membrane pores, and hollow-arrows indicate cells that had migrated through the membrane, which were stained with Crystal Violet (a). Cell migration of the Mia ez22 (b), Mia pcb6 (a), Mia ezsi-E (e) and Mia ezsi-cram (d) cells after 12 hours were shown. The cells migrating to the lower chambers were analyzed. For quantification, the cells were counted in 10 random fields under a light microscope (×400). Compared to the Mia pcb6 cells, the Mia ez22-B cells showed a significant increase in migration by x²-test (c). The decrease in the numbers of migrated cells in the Mia ezsi-E cells compared to those of the Mia ezsi-scram cells was statistically significant, shown by the x²-test (f). Columns: mean; bars: SD.