Figure 3
From: Ezrin promotes invasion and metastasis of pancreatic cancer cells
Effects of ezrin on MiaPaCa-2 cell growth and anchorage-independent growth. (A) The cell growth curves of the Mia ezsi-scram, Mia ezsi-E, Mia pcb6 and Mia ez22-B cells were assayed on days 1-7. (B) Flow cytometry assay showing the percentage of different cell cycle phases in the four cell clones. (C) Anchorage-independent growth assay of ezrin-overexpressing and ezrin-silencing cells. The cell growth ability in soft agar of the four cell clones was examined for three weeks. Columns, mean; bars, SD. (D) Statistical analysis of colony formation in the four cell clones. There was a significant difference of the colony formation ability between the Mia ez22-B and the Mia pcb6 cells, as well as between the Mia ezsi-scram and the Mia ezsi-E cells, respectively, shown by x2-test. The results are expressed as the mean ± SD of three independent experiments.