Figure 1

Stable overexpression and silencing of ezrin in MiaPaCa-2 cells. (A) Western blot showed the ezrin protein was overexpressed in the Mia ez22-B cells compared to the Mia pcb6 cells using an ezrin antibody. The relative ezrin protein level was quantified by densitometry analysis. The ezrin protein was efficiently increased by 3.8 folds in the Mia ez22-B cells. (B) Ectopic expression of ezrin in the Mia ez22-B cells was detected using a VSV-G antibody (VSV-G tag in the pcb6-ezrin vector). (C) The expression level of ezrin protein was dramatically decreased by 70.5% and 90.1% in the Mia ezsi-B and the Mia ezsi-E cells, respectively, compared to that in the Mia ezsi-scram cells. GAPDH was used as a loading control. (D) The Mia pcb6 cells were stained with the ezrin antibody and a FITC-conjugated second antibody to detect the ezrin protein expression. (E) The vector tag VSV-G antibody and a Rhodamine-conjugated second antibody were used to detect the exogenous ezrin protein expression. (F, G) The Mia ezsi-scram and the Mia ezsi-E cells were stained with the ezrin antibody and the FITC-conjugated second antibody to detect the ezrin protein expression.