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Table 1 Gene expression by qPCR in resting or LPS-activated IRAK4-deficient monocytes relative to gene expression in unstimulated control cells

From: Robust TLR4-induced gene expression patterns are not an accurate indicator of human immunity

Gene Name Monocytes Resting   LPS  
DEFB1 Control a1.1 ± 0.6   12.6 ± .0  
  up =    
  IRAK4 Q293X 1.9 ± 0.9   9.6 ± 0.9  
Fos Control 1.5 ± 0.1   0.3 ± 0.0  
  = =    
  IRAK4 Q293X 1.3 ± 0.0   0.3 ± 0.1  
Gro-α (CXCL1) Control 1.0 ± 0.2   6.3 ± 1.2  
  dn dn    
  IRAK4 Q293X 0.3 ± 0.0   0.3 ± 0.0  
IL-6 Control 1.1 ± 0.6   185.4 ± 110.0  
  dn dn    
  IRAK4 Q293X 0.4 ± 0.5   1.8 ± 2.1  
IL-8 Control 1.0 ± 0.0   24.1 ± 5.5  
  = dn    
  IRAK4 Q293X 0.9 ± 0.8   2.0 ± 1.4  
IL-12β Control 1.0 ± 0.4   181.7 ± 8.0  
  dn dn    
  IRAK4 Q293X 0.2 ± 0.1   1.9 ± 0.8  
IL-13RA1 Control 1.0 ± 0.1   0.3 ± 0.0  
  = up    
  IRAK4 Q293X 1.1 ± 0.3   1.9 ± 0.1  
IFN-γ Control 1.0 ± 0.1   11.0 ± 0.3  
  dn dn    
  IRAK4 Q293X 0.4 ± 0.1   0.8 ± 0.3  
IRF1 Control 1.0 ± 0.1   6.7 ± 0.1  
  dn dn    
  IRAK4 Q293X 0.6 ± 0.1   3.1 ± 0.2  
IκBζ Control 1.1 ± 0.5   1.3 ± 0.3  
  dn dn    
  IRAK4 Q293X 0.4 ± 0.2   0.5 ± 0.1  
Jun Control 1.0 ± 0.0   0.9 ± 0.2  
  = up    
  IRAK4 Q293X 0.9 ± 0.3   1.7 ± 0.4  
MCP-2 (CCL8) Control 1.0 ± 0.0   9.4 ± 0.0  
  dn dn    
  IRAK4 Q293X 0.1 ± 0.0   1.8 ± 0.2  
MDC (CCL22) Control 1.1 ± 0.5   4.1 ± 1.2  
  dn up    
  IRAK4 Q293X 0.6 ± 0.2   6.4 ± 1.8  
MIP-3α (CCL20) Control 1.0 ± 0.2   83.9 ± 0.0  
  dn dn    
  IRAK4 Q293X 0.1 ± 0.0   1.4 ± 0.5  
NFκB1 (p105/p50) Control 1.0 ± 0.1   6.8 ± 0.1  
  dn dn    
  IRAK4 Q293X 0.3 ± 0.1   2.3 ± 0.7  
NFκB2 (p100/p52) Control 1.0 ± 0.0   4.5 ± 0.7  
  dn dn    
  IRAK4 Q293X 0.7 ± 0.0   1.9 ± 0.3  
NFκB cRel Control 1.0 ± 0.1   3.8 ± 0.1  
  dn dn    
  IRAK4 Q293X 0.7 ± 0.0   1.0 ± 0.0  
NFκB RelA (p65) Control 1.0 ± 0.0   3.3 ± 0.2  
  = dn    
  IRAK4 Q293X 1.2 ± 0.0   2.3 ± 0.4  
NFκB RelB Control 1.0 ± 0.2   1.9 ± 0.4  
  = =    
  IRAK4 Q293X 0.8 ± 0.0   1.7 ± 0.3  
SOCS1 Control 1.2 ± 0.8   21.2 ± 4.7  
  dn dn    
  IRAK4 Q293X 0.1 ± 0.1   1.6 ± 0.5  
TNF-α Control 1.0 ± 0.1   11.3 ± 4.0  
  = dn    
  IRAK4 Q293X 0.9 ± 0.6   2.4 ± 0.7  
ZFP36L2 Control 1.0 ± 0.1   0.8 ± 0.0  
  up up    
  IRAK4 Q293X 2.1 ± 0.5   2.1 ± 0.2  
  1. a fold changes normalized to endogenous GAPDH in patient or control cells then expressed relative to that in the pool of unstimulated control monocytes (fold change = 1.0) ± the standard deviation of 2 biological and 2 technical replicates.