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Table 1 Gene expression by qPCR in resting or LPS-activated IRAK4-deficient monocytes relative to gene expression in unstimulated control cells

From: Robust TLR4-induced gene expression patterns are not an accurate indicator of human immunity

Gene Name

Monocytes

Resting

 

LPS

 

DEFB1

Control

a1.1 ± 0.6

 

12.6 ± .0

 
 

up

=

   
 

IRAK4 Q293X

1.9 ± 0.9

 

9.6 ± 0.9

 

Fos

Control

1.5 ± 0.1

 

0.3 ± 0.0

 
 

=

=

   
 

IRAK4 Q293X

1.3 ± 0.0

 

0.3 ± 0.1

 

Gro-α (CXCL1)

Control

1.0 ± 0.2

 

6.3 ± 1.2

 
 

dn

dn

   
 

IRAK4 Q293X

0.3 ± 0.0

 

0.3 ± 0.0

 

IL-6

Control

1.1 ± 0.6

 

185.4 ± 110.0

 
 

dn

dn

   
 

IRAK4 Q293X

0.4 ± 0.5

 

1.8 ± 2.1

 

IL-8

Control

1.0 ± 0.0

 

24.1 ± 5.5

 
 

=

dn

   
 

IRAK4 Q293X

0.9 ± 0.8

 

2.0 ± 1.4

 

IL-12β

Control

1.0 ± 0.4

 

181.7 ± 8.0

 
 

dn

dn

   
 

IRAK4 Q293X

0.2 ± 0.1

 

1.9 ± 0.8

 

IL-13RA1

Control

1.0 ± 0.1

 

0.3 ± 0.0

 
 

=

up

   
 

IRAK4 Q293X

1.1 ± 0.3

 

1.9 ± 0.1

 

IFN-γ

Control

1.0 ± 0.1

 

11.0 ± 0.3

 
 

dn

dn

   
 

IRAK4 Q293X

0.4 ± 0.1

 

0.8 ± 0.3

 

IRF1

Control

1.0 ± 0.1

 

6.7 ± 0.1

 
 

dn

dn

   
 

IRAK4 Q293X

0.6 ± 0.1

 

3.1 ± 0.2

 

IκBζ

Control

1.1 ± 0.5

 

1.3 ± 0.3

 
 

dn

dn

   
 

IRAK4 Q293X

0.4 ± 0.2

 

0.5 ± 0.1

 

Jun

Control

1.0 ± 0.0

 

0.9 ± 0.2

 
 

=

up

   
 

IRAK4 Q293X

0.9 ± 0.3

 

1.7 ± 0.4

 

MCP-2 (CCL8)

Control

1.0 ± 0.0

 

9.4 ± 0.0

 
 

dn

dn

   
 

IRAK4 Q293X

0.1 ± 0.0

 

1.8 ± 0.2

 

MDC (CCL22)

Control

1.1 ± 0.5

 

4.1 ± 1.2

 
 

dn

up

   
 

IRAK4 Q293X

0.6 ± 0.2

 

6.4 ± 1.8

 

MIP-3α (CCL20)

Control

1.0 ± 0.2

 

83.9 ± 0.0

 
 

dn

dn

   
 

IRAK4 Q293X

0.1 ± 0.0

 

1.4 ± 0.5

 

NFκB1 (p105/p50)

Control

1.0 ± 0.1

 

6.8 ± 0.1

 
 

dn

dn

   
 

IRAK4 Q293X

0.3 ± 0.1

 

2.3 ± 0.7

 

NFκB2 (p100/p52)

Control

1.0 ± 0.0

 

4.5 ± 0.7

 
 

dn

dn

   
 

IRAK4 Q293X

0.7 ± 0.0

 

1.9 ± 0.3

 

NFκB cRel

Control

1.0 ± 0.1

 

3.8 ± 0.1

 
 

dn

dn

   
 

IRAK4 Q293X

0.7 ± 0.0

 

1.0 ± 0.0

 

NFκB RelA (p65)

Control

1.0 ± 0.0

 

3.3 ± 0.2

 
 

=

dn

   
 

IRAK4 Q293X

1.2 ± 0.0

 

2.3 ± 0.4

 

NFκB RelB

Control

1.0 ± 0.2

 

1.9 ± 0.4

 
 

=

=

   
 

IRAK4 Q293X

0.8 ± 0.0

 

1.7 ± 0.3

 

SOCS1

Control

1.2 ± 0.8

 

21.2 ± 4.7

 
 

dn

dn

   
 

IRAK4 Q293X

0.1 ± 0.1

 

1.6 ± 0.5

 

TNF-α

Control

1.0 ± 0.1

 

11.3 ± 4.0

 
 

=

dn

   
 

IRAK4 Q293X

0.9 ± 0.6

 

2.4 ± 0.7

 

ZFP36L2

Control

1.0 ± 0.1

 

0.8 ± 0.0

 
 

up

up

   
 

IRAK4 Q293X

2.1 ± 0.5

 

2.1 ± 0.2

 
  1. a fold changes normalized to endogenous GAPDH in patient or control cells then expressed relative to that in the pool of unstimulated control monocytes (fold change = 1.0) ± the standard deviation of 2 biological and 2 technical replicates.