Figure 4
From: Robust TLR4-induced gene expression patterns are not an accurate indicator of human immunity
Strong expression of particular chemokines in IRAK4-deficient monocytes. The relative expression (Y-axis) of (A) DEFB1 and (B) MDC/CCL22 in IRAK4-deficient monocytes (black bars), controls (white bars) and for B) CD14- PBMC. Expression was measured in two independent experiments by real-time PCR following 4 hr incubation in the absence or presence of LPS (X-axis). C MDC/CCL22 in the tissue culture supernatant was measured by ELISA in IRAK4-deficient (black bars), adult (white bars) or age-matched (grey bars) PBMC. The result is representative of 2 independent experiments and the y-axis represents the relative fold change in protein after stimulation for 24 hr with LPS.