Figure 3
From: Robust TLR4-induced gene expression patterns are not an accurate indicator of human immunity
Expression of inflammatory cytokines and chemokines in IRAK4-deficient monocytes. A Expression of IL-6, IL-8, IL-12β, TNF-α, Gro-α/CXCL1 and MIP-3α/CCL20 in the healthy control pooled monocytes (white bars) and in both biological replicates of the patient's monocytes (black bars) were evaluated, in duplicate, by qPCR following a 4 hr incubation in the absence or presence of LPS (X-axis). B IL-6, IL-8 and TNF-α were also measured in the tissue culture supernatant of stimulated PBMC by multiplex cytokine bead immunoassays. The Y-axis represents fold change (log scale) in gene expression relative to unstimulated control monocytes (qPCR) or pg/ml of protein determined by the cytokine assay. Differences in expression or protein secretion were determined to be statistically significant by Student's two tailed T-test with a value of p < 0.15 (*), p < 0.1 (**), p < 0.05 (***) and p < 0.003 (****).