Figure 1

Clotting characterization 50 uL fibrin clots were prepared with 25 uL of fibrinogen and 25 uL of thrombin at various concentrations of both. A: Clotting test on 28 fibrin formulations. Positive clotting was defined as the formation of a solid and homogenous clot. The dotted line shows the immobilization zone, where proper clotting was attained. B: Clot stability test for five selected formulas from the immobilization zone. The clots were cultured in regular cell culture media or in conditioned media in 24 well plates at 37°C. After 24 and 72 hours, clot samples were taken (n = 3) and visually examined to determine fibrin crumbling.