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Table 1 Quantification of the relative amounts of the three htid splice forms L, I and S, and the HER-2 transcript in human normal breast epithelium

From: In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor

Target gene/splice form htid-L htid-I htid-S erbB-2
Samples/ΔCt-value 4,11 1,54 15,82 -2,75
  3,17 0,59 15,38 -1,96
  3,53 0,57 13,69 -2,26
  3,78 0,84 14,95  
  4,12 1,51 14,87  
average ΔCt (=100%) 3,74 1,01 14,94 -2,32
± 0,3 (8,00%) ± 0,4 (39,61%) ± 0,5 (3,35%) ± 0,29 (12,50%)
Amount of target: 2 -ΔCt 0,07 0,50 3.18 x 10 -5 4,99
  1. The ΔCt value is determined by substracting the HGPRT (endogenous reference) Ct-value from the Ct value of the target investigated: ΔCt = Ct (target) - Ct (HGPRT); Standard deviation (SD): , n = 3, was calculated for the average ΔCt (100%); The relative concentration of the target (amount of target), 2-ΔCt, is calculated for the average ΔCt values.