Table 1 Quantification of the relative amounts of the three htid splice forms L, I and S, and the HER-2 transcript in human normal breast epithelium
Target gene/splice form | htid-L | htid-I | htid-S | erbB-2 |
|---|---|---|---|---|
Samples/ΔCt-value | 4,11 | 1,54 | 15,82 | -2,75 |
3,17 | 0,59 | 15,38 | -1,96 | |
3,53 | 0,57 | 13,69 | -2,26 | |
3,78 | 0,84 | 14,95 | ||
4,12 | 1,51 | 14,87 | ||
average ΔCt (=100%) | 3,74 | 1,01 | 14,94 | -2,32 |
| ± 0,3 (8,00%) | ± 0,4 (39,61%) | ± 0,5 (3,35%) | ± 0,29 (12,50%) |
Amount of target: 2 -ΔCt | 0,07 | 0,50 | 3.18 x 10 -5 | 4,99 |
- The ΔCt value is determined by substracting the HGPRT (endogenous reference) Ct-value from the Ct value of the target investigated: ΔCt = Ct (target) - Ct (HGPRT); Standard deviation (SD):
, n = 3, was calculated for the average ΔCt (100%); The relative concentration of the target (amount of target), 2-ΔCt, is calculated for the average ΔCt values.