Figure 2
MHC class II restricted T-cell responses to Her2 before and after Her2-pDNA vaccination. A-D. Her2-specific IFN-γ production by T-cells from patients #3, 4, 7 and 8, before (3 days pre-) and after (10 days post-) Her2-pDNA vaccination and at long term follow-up (41, 38.5 and 22 month after last vaccination for patients #3, 4, 8, respectively). Bars show mean (± s.e.m.) frequency of IFN-γ producing T-cells (spot forming units) per 2.5 × 105 PBMC responding to a panel of 4 degenerate Her2-derived HLA-DR epitopes (p59, p88, p422 and p885). No pre-bleeding ELISpot was performed for patient 3 due to insufficient numbers of available PBMC. E. Mean ± s.e.m. Her2-specific T-cell frequency per 2.5 × 105 PBMC in patients evaluable at all time points. Bars show pooled responses of patients #4 and 8 pre, post and late. *: p ≤ 0.05.