FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy

Table 1 mDC FRET efficiency measured 16 hr and 46 hr after maturation stimulus

mDC 16 hr	ROI 1	ROI 2	ROI 3	ROI 4	ROI 5	ROI 6
Donor Pre	33	23	26	25	29	46
Donor Post	34	23	25	26	28	45
Acceptor Pre	46	59	60	47	35	74
Acceptor Post	8	8	7	6	33	72
E (%)	2.94	0	0	3.85	0	0
mDC 46 hr	ROI 1	ROI 2	ROI 3	ROI 4	ROI 5	ROI 6
Donor Pre	31	45	43	41	37	44
Donor Post	36	62	52	49	37	42
Acceptor Pre	56	142	132	142	139	133
Acceptor Post	7	9	8	8	141	139
E (%)	13.9	27.4	17.3	16.3	0	0

ROI, Region of interest; Pre, before photobleaching; Post, after photobleaching; mDC 16 or 46 hr, Dendritic Cells matured for 16 or 46 hr; E (%), Energy Transfer Efficiency. The data shown refer to six significant ROIs of the experiments reported in Figure 1A. ROI1 to ROI4 represent regions selected within the bleached area, whereas ROI5 and ROI6 represent regions selected outside the bleached area, which were used as controls. The numbers in the "Donor" and the "Acceptor" rows indicate the fluorescence intensity levels detected in each ROI.

ISSN: 1479-5876