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Table 1 mDC FRET efficiency measured 16 hr and 46 hr after maturation stimulus

From: FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy

mDC 16 hr ROI 1 ROI 2 ROI 3 ROI 4 ROI 5 ROI 6
Donor Pre 33 23 26 25 29 46
Donor Post 34 23 25 26 28 45
Acceptor Pre 46 59 60 47 35 74
Acceptor Post 8 8 7 6 33 72
E (%) 2.94 0 0 3.85 0 0
mDC 46 hr ROI 1 ROI 2 ROI 3 ROI 4 ROI 5 ROI 6
Donor Pre 31 45 43 41 37 44
Donor Post 36 62 52 49 37 42
Acceptor Pre 56 142 132 142 139 133
Acceptor Post 7 9 8 8 141 139
E (%) 13.9 27.4 17.3 16.3 0 0
  1. ROI, Region of interest; Pre, before photobleaching; Post, after photobleaching; mDC 16 or 46 hr, Dendritic Cells matured for 16 or 46 hr; E (%), Energy Transfer Efficiency. The data shown refer to six significant ROIs of the experiments reported in Figure 1A. ROI1 to ROI4 represent regions selected within the bleached area, whereas ROI5 and ROI6 represent regions selected outside the bleached area, which were used as controls. The numbers in the "Donor" and the "Acceptor" rows indicate the fluorescence intensity levels detected in each ROI.