FRET measurements. (A) Averaged FRET efficiency of mDC as a function of time from the maturation stimulus. The data and the Standard Errors (±SE) refer to FRET measurements performed over at least three fields for each sample (n = 3-5) and different ROIs (n = 30-55) inside the bleached regions. The x axis displays the time in culture after maturation stimulus. (B) Plot of the independence of E% from acceptor levels. The data shown were generated from image measurements 22 hr after maturation. The acceptor levels refer to the intensity of the image acquired before acceptor photobleaching and analyzed versus the recovered E%, on a pixel by pixel basis.