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Table 5 rhIL21 induced IL2RA expression in whole blood from cynomolgus monkeys dosed with Ab-01

From: Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

Animal Treatment Group Time point Ex vivoIL2RA Fold Change Response to IL21 Stimulation Cpeak
1 Ab-01 pre-dose 2.8 not applicable
1 Ab-01 5 minute post dose 0.8 200
2 Ab-01 pre-dose 4.8 not applicable
2 Ab-01 5 minute post dose 0.8 139
3 Ab-01 pre-dose 4.2 not applicable
3 Ab-01 5 minute post dose 0.9 153
4 Control IgG1TM pre-dose 4.2 not applicable
4 Control IgG1TM 5 minute post dose 4.3 not applicable
5 Control IgG1TM pre-dose 2.7 not applicable
5 Control IgG1TM 5 minute post dose 4.4 not applicable
6 Control IgG1TM pre-dose 9.1 not applicable
6 Control IgG1TM 5 minute post dose 7.7 not applicable
  1. Relative RNA concentration of IL2RA induced by ex vivo addition of rhIL-21 to whole blood obtained from cynomolgus monkeys before and after treatment with either Ab-01 or control IgG1TM is shown. Pre-dose whole blood sample was taken on day -13 for animal 1, and on day 0 (the day of dosing) for all others. All monkeys were dosed with 10 mg/kg via intravenous (IV) route. The ex vivo response to IL21 was completely blocked in monkeys treated with Ab-01 and was not blocked in control IgG1TM treated monkeys