Figure 4
From: Overexpression of microRNA-206 in the skeletal muscle from myotonic dystrophy type 1 patients
In situ hybridisation showing miR-206 localization in transversal section of vastus lateralis muscle from one DM1 patient and one control subject. 4a: Tissue distribution of miR-206 in an healthy subject. miR-206 was expressed mostly in nuclear regions. 4b: Tissue distribuition of miR-206 in a DM1 patient. The miR-206 strongest signal corresponds to nuclear clumps (red arrow). Expression of miR-206 was also observed in nuclear regions of centralized nuclei. 4c: hybridization of U6-siRNA LNA used as positive control. 4d: LNA probe with a scrambled sequence, which is not present in the human genome, has been used to test the specificity of the probes. Green signal corresponds to lipofuscin-derived autofluorescence of the muscle tissue and does not localize with the nuclei.