Northern blot analysis of myo-miRs expression. DM1 patients were pooled into two groups: DMa (DM1-1, DM1-2, DM1-3, DM1-4) and DMb (DM1-5, DM1-6, DM1-7), 4 healthly subjects were pooled as well. The U6-snRNA was used as control for normalization of samples. Figure 2a: Northern Blot results of the 3 pooled samples (Ctr, DMa and DMb) for the 4 miRNA analyzed (miR 181, miR 1, miR 206 and miR 133) compared to U6-snRNA. Figure 2b: Densitometry of autoradiograms performed using OptiQuant image analysis software (Packard) showing miR/U6 ratios.