Figure 3

HSF1 overexpression increases the replication of Adel55 in Bcap37 cells. (A) Replication of Adel55 in the indicated cell lines. 10⁵ cells were plated into six-well plates. 24 h later, cells were infected with 10⁴ PFU of Adel55. After incubation for 3, 5, or 7 days, whole medium and cells extracts were prepared and titrated for virus production by standard plaque assay on 293 cells. The data represent the mean ± SD of four repeats. (B) The replication of Adel55-EGFP. Indicated cell lines were infected with Adel55-EGFP at MOI of 0.1. 3 or 5 days later, EGFP expression was detected under fluorescence microscopy (top panel). The corresponding cell death could be observed under bright field microscopy (bottom panel).