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Table 2 Comparison of photodynamic effects in BMG-1 cells pre-incubated with different concentrations of AlPcS2.

From: Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line

Endpointa Time after Photo- irradiation 0.0 μM 1.0 μM 5.0 μM 10.0 μM
Intracellular content of AlPcS2 (pg/cell) (absorbance) 2 h 0.0 1.9 ± 0.05 1.9 ± 0.06 2.9 ± 0.05
Relative fluorescence intensity 2 h 0.0 92 ± 13.0 105 ± 10.0 165 ± 9.0
Fluorescence Distribution 2 h - Perinuclear and Granular in cytoplasm Diffuse in cytoplasm Diffuse in cytoplasm but membrane damage in some cells
Clonogenic Survival 240 h 1.0 ± 0.0 0.53 ± 0.07 0.65 ± 0.1 0.98 ± 0.22
Proliferation Indexb 42 h 5.6 ± 0.4 3.5 ± 0.5 1.0 ± 0.4 NDc
G2+M (%) 42 h 7.0 ± 2.0 12.0 ± 2.0 23.0 ± 0.8 ND
Detached Cells (%) 42 h 19.0 ± 2.8 15.0 ± 0.3 30.0 ± 8.0 ND
Small cells (%) 42 h 4.0 ± 0.1 24.0 ± 0.2 57.0 ± 0.1 ND
Sub G0/G1 (%) 42 h 0.0 3.0 ± 0.2 23.0 ± 10.0 ND
M. F. (%) 42 h 1.6 ± 0.2 1.5 ± 0.1 1.7 ± 0.5 ND
  1. aEndpoints are summarized as mean ± standard deviation by groups.
  2. bNt/N0 Where Nt is the cell number at 42 h and N0 is the cell numbers at the time of irradiation.
  3. cND- not determined.