Table 2 Comparison of photodynamic effects in BMG-1 cells pre-incubated with different concentrations of AlPcS₂.

Intracellular content of AlPcS₂ (pg/cell) (absorbance)

2 h

0.0

1.9 ± 0.05

1.9 ± 0.06

2.9 ± 0.05

Relative fluorescence intensity

2 h

0.0

92 ± 13.0

105 ± 10.0

165 ± 9.0

Fluorescence Distribution

2 h

-

Perinuclear and Granular in cytoplasm

Diffuse in cytoplasm

Diffuse in cytoplasm but membrane damage in some cells

Clonogenic Survival

240 h

1.0 ± 0.0

0.53 ± 0.07

0.65 ± 0.1

0.98 ± 0.22

Proliferation Index^b

42 h

5.6 ± 0.4

3.5 ± 0.5

1.0 ± 0.4

ND^c

G₂+M (%)

42 h

7.0 ± 2.0

12.0 ± 2.0

23.0 ± 0.8

ND

Detached Cells (%)

42 h

19.0 ± 2.8

15.0 ± 0.3

30.0 ± 8.0

ND

Small cells (%)

42 h

4.0 ± 0.1

24.0 ± 0.2

57.0 ± 0.1

ND

Sub G₀/G₁ (%)

42 h

0.0

3.0 ± 0.2

23.0 ± 10.0

ND

M. F. (%)

42 h

1.6 ± 0.2

1.5 ± 0.1

1.7 ± 0.5

ND