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Figure 4 | Journal of Translational Medicine

Figure 4

From: Correlation of pharmacodynamic activity, pharmacokinetics, and anti-product antibody responses to anti-IL-21R antibody therapeutics following IV administration to cynomolgus monkeys

Figure 4

Correlation of serum concentrations, PD activity, and anti-product antibody responses following a single 10 mg/kg IV dosage of anti-IL-21R antibody Ab-02 to cynomolgus monkeys. Each pre-dose (day 1) and post-dose whole blood sample was divided into four 1.5 mL aliquots. First and second aliquots were treated with either rhuIL-21 (filled circle) or vehicle (a calibrator for RQ calculations), respectively, and were used to assess whether circulating test article affected ex vivo rhuIL-21-induced IL-2RA gene expression (i.e. PD activity). The third aliquot (filled square) was treated with rhuIL-21 and Ab-02 and the fourth aliquot (open triangle) was treated with rhuIL-21 and an IgG control antibody (negative control for the third treatment). Ab-02 serum concentrations (filled diamonds) were measured by a specific ELISA up to day 36. Data points with serum concentrations below the LOQ (30 ng/mL) are not shown. Neutralizing anti-Ab-02 antibodies were assessed by flow cytometry; positive result indicated by "AN".

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