miR expression changes in maturing DCs. Immature DCs from 6 healthy subjects were incubated with LPS and IFN-γ and after 0, 4, 8, and 24 hours of culture, they were analyzed by global microRNA expression profiling using a microarray with 827 probes. The differentially expressed human miR (F-test: p < 0.05) were analyzed by supervised hierachical clustering. The samples clustered into 4 groups based on maturation time, iDC are indicated by the green bar, DCs cultured for 4 hours by the orange bar, DCs cultured for 8 hours by the red bar, and 24 hours by the purple bar. The miRs sorted into 2 separate clusters and miRs from each of the clusters are shown.