Gene expression changes in maturing DCs. Immature DCs from 6 healthy subjects were incubated with LPS and IFN-γ. After 0, 4, 8, and 24 hours of culture, DCs were analyzed by gene expression profiling using a microarray with 35,035 oligonucleotide probes. The 2,370 differentially expressed genes (F-test: p < 0.001) were analyzed by supervised hierachical clustering. Immature DCs are indicated by the orange bar, iDCs cultured with LPS and IL-4 for 4 hours by the green bar, 8 hours by the purple bar, and 24 hours by the red bar. The genes sorted into 5 separate clusters and representive genes from each of the 5 clusters are shown.