Table 1 Genomic characterization, growth kinetics and sensitivity towards PLX4032 for a panel of human melanoma cell lines.
Cell Line | NRAS/BRAF | Number of BRAF Gene Copies | Other Oncogenic Events | Cell line doubling time (hours) | PLX4032 IC50 (μM) |
|---|---|---|---|---|---|
M257 | Wild type | 3 | CDKN2A R80 | 31.4 | Not reached |
M202 | NRAS Q61L | 2 | EGFR amplification CDKN2A homozygous deletion | 26.1 | Not reached |
M207 | NRAS Q61L | 2 | MITF amplification PTEN heterozygous deletion | 25.2 | Not reached |
M233 | BRAFV600E Heterozygous | 3 | AKT1 amplification CCND1 amplification EGFR amplification CDKN2A homozygous deletion PTEN homozygous deletion | 29.6 | Not reached |
M255 | BRAFV600E Heterozygous | 2 | AKT2 amplification CCND1 amplification EGFR amplification CDKN2A homozygous deletion | 48.6 | Not reached |
M308 | BRAFV600E Heterozygous | 3 | MITF amplification AKT2 amplification EGFR amplification CDKN2A heterozygous deletion | 35.0 | Not reached |
M263 | BRAFV600E Heterozygous | 2 | CDKN2A heterozygous deletion | 23.3 | 10 |
M321 | BRAFV600E Homozygous | 2 | 34.1 | 7.5 | |
SKMEL28 | BRAFV600E Homozygous | 2 | EGFR P753S MITF amplification CCND1 amplification CDKN2A heterozygous deletion PTEN heterozygous deletion | 26.9 | 4.6 |
M229 | BRAFV600E Homozygous | 4 | MITF amplification AKT1 amplification PTEN heterozygous deletion | 27.9 | 0.2 |
M238 | BRAFV600E Heterozygous | 2 | CDKN2A homozygous deletion PTEN heterozygous deletion | 28.1 | 0.7 |
M249 | BRAFV600E Heterozygous | 3 | MITF amplification AKT2 amplification PTEN homozygous deletion | 21.2 | 0.8 |
M262 | BRAFV600E Homozygous | 2 | AKT1 E17K AKT1 amplification EGFR amplification CDKN2A homozygous deletion | 47.4 | 0.1 |