Figure 3

Western blot analysis of phosphorylated and total amount of key proteins in the MAPK and PI3k/Akt pathways. a) The PLX4032-sensitive M229 cell line and the PLX4032-resistant M233 cell line were cultured in different concentrations of PLX4032 for 24 hours and lysates were analyzed by Western blot. b) M229 and M233 cells were treated by PLX4032 in a time course over 24 hours, and cell lysates were analyzed by Western blot.