MiRNA-449a/b expression under hypoxia. Primary human fibroblasts were transfected with miRNA-449a/b inhibitor or miRNA-449a/b mimics and were cultured under hypoxia. Inhibition or mimicking strongly decreased or increased miRNA-449a/b expression, respectively. Depicted are calculations relative to reference gene RNU48 compared with non transfected cells cultured with hypoxia. Negative control means cells transfected with negative control siRNA only. Results are shown for cell line F-18 but were likewise demonstrable in M15D. * = p < 0.05 (three independent experiments).