Figure 5

Immunohistochemistry for LMP1, p-mTOR, p-P70S6K and p-4EBP1 in NPC biopsies. Two representative NPC tissues are shown (200×), one in A to E, and another in frame F to J. Frames A and F are hematoxylin-eosin (H&E) staining. Positive immunostaining of LMP1 in the membrane and cytoplasm is in frame B, and expression of p-mTOR, p-P70S6K and p-4EBP1 with cytoplasm staining is in C, D, E, respectively. Samples with strong LMP1 expression also presented high levels of p-mTOR, p-P70S6K and p-4EBP1 (B, C, D and E), while samples with no LMP1 expression showed very weak or no expression of p-mTOR, p-P70S6K and p-4EBP1 (G, H, I and J).