Figure 4

LMP1 silencing reduced p-mTOR and p-4EBP1 expression in the C666-1 line. C666-1 cells were harvested for immunofluoresence after 72 h LMP1-siRNA transfection at a final concentration of 50 or 100 nm. Negative control (NC)-siRNA acted as an internal standard. LMP1 expression was in the membrane and cytoplasm, with p-mTOR and p-4EBP1. The location of LMP1 and p-mTOR or p-4EBP1 in the C666-1 cell line overlapped perfectly. When LMP1 expression was decreased with 50 nm siRNA in C666-1 cells, expression of p-mTOR and p-4EBP1 was also reduced. No detectable p-mTOR or p-4EBP1 expression was observed when LMP1 was completely silenced by 100 nm siRNA in C666-1 cells.