Figure 1

LMP1 regulates a number of genes expressed in the NPC cell line HONE1-LMP1. A. Western blot of cell lysates from HONE1-LMP1 and HONE1-vector cell lines showing that LMP1 upregulated p-IκBα, p-NF-κB, PARP and Survivin, and downregulated PTEN. GAPDH was a loading control. B. Changes in gene expression measured by densitometry. LMP1 increased five-fold in the HONE1-LMP1 cell line. Compared to the HONE1-vector cell line, the expression of p-IκBα and p-NF-κB increased almost 2.5-fold and a 1.4-fold increase was observed in PARP and Survivin in the HONE-LMP1 cell line. PTEN expression was reduced by half in the HONE1-LMP1 line compared to the HONE1-vector line.