Figure 1

Microarray analysis demonstrates up-regulation of miR-17-92 in Th1 cells. (A), Intracellular IFN-γ vs. IL-4 expression of Th1 and Th2 cells induced from mouse CD4⁺ splenic T cells in vitro. (B), Differentially expressed miRs were analyzed by hierarchical clustering of the log2 value of Th1/Th2 pair of miR microarray signal. Red indicates up-regulation in Th1; green, up-regulation in Th2. (C), miRs were ranked by relative fold expression in Th1/Th2 cells. Arrows indicate members of the miR-17-92 cluster or paralog clusters. miRs with a relative expression of >2.35 fold in Th1 are shown. (B and C), hsa- and mmu- indicate human and mouse miR probes, respectively. Hsa-probes can hybridize with most mouse miR due to the high homology and mmu-signals are shown only when murine miR has unique sequence compared to its human counterpart. (D), Ideogram of mouse chromosome 14 showing the location and order of the miR-17-92 cluster (adapted from NCBI Blast).