CD14 mRNA expression in liver tissues of patients with BA. In situ hybridization of CD14 mRNA in the livers of patients with early- and late-stage BA. CD14 is stained brown by in situ hybridization with a DIG-labeled CD14 sense (A-C) and antisense RNA probe (D-F). The paraffin-embedded sections from patients with hepatoblastoma as control (A, D), early-stage BA (B, E) and late-stage BA (C, F) Tissues were counterstained with hematoxylin. The CD14 mRNA expression pattern mainly localized in the hepatocytes (arrowhead) and the bile duct epithelial cells (arrow). Original magnification: × 200.