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Figure 1 | Journal of Translational Medicine

Figure 1

From: Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation

Figure 1

Immunorecognition of hGSN by three antibodies in Western blot assay. (A) The location of epitope specific to the mouse, goat and rabbit anti-hGSN antibodies are provided in reference to the full-length pGSN. Numbers above each epitope correspond to the amino acid sequence from the full-length (with signal sequence intact) pGSN containing peptides used as antigens. (B) Summary of Western blot analyses revealing that immunoreactivity of pGSN depends on not only antibodies but also source of antigen. Total protein loaded per source per lane: 25 μg of human plasma from HIV-infected individuals, 10 μg of human CSF from HIV-infected individuals, 20 μg of cell supernate from both HIV-infected and non-infected cells, 25 μg of monkey plasma from pre- and 10 days post-infection of rhesus macaques with SIV, and 2 μg each of commercially available gelsolin. Membranes from each source were probed with mouse anti-hGSN, goat anti-hGSN, and rabbit anti-hGSN (all 1:1000) and corresponding HRP-conjugated secondary antibodies (1:20,000) diluted in PBS supplemented with 10% Tween-20 and 10% (w/v) skim milk.

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