Gene silencing of ADAM 10 reduces tumor metastasis in vivo. (a) Overview of lung tissues from mice injected with the indicated cell lines (scale bar = 0.5 cm). Tumors are indicated by black arrows. (b) Immunohistochemical staining of ADAM 10 from tumors derived from injected SACC-LM cells (scale bar = 50 μm). (c) Lung weight. (d) Quantification of immunohistochemical staining of ADAM 10 from b using Image Pro Plus software (*p < 0.001 compared with SACC-LM). SACC-LM (high metastatic potential control); SACC-mock (mock transfection control); SACC-scrambled RNA (scrambled siRNA control); SACC-ADAM 10-RNAi (1), (2), and (3) represent the three different clones, respectively.