Gene silencing of ADAM 10 reduces cell proliferation and migration in SACC-LM cells. (a) A Matrigel transwell invasion assay was used to test the ability of the indicated cell lines to invade the filter membrane. (b) Values represent the cell number (mean ± SD) per visible field (*p < 0.001 compared with SACC-LM). (c) Cell proliferation was analyzed using the MTT assay. Cells were monitored for 8 days and the average OD490 (± SD) for each cell line is shown. Cells transfected with ADAM 10 siRNA showed reduced cell growth relative to parental and mock-transfected cells. SACC-LM (high metastatic potential control); SACC-Mock (mock transfection control); SACC-ADAM10-RNAi (1), (2), and (3) represent the three different clones, respectively.