Figure 4

The expression of H19 and IGF2-P4 in heterotopic subcutaneous tumors determined by RT-PCR: The expression of H19 and IGF2-P4 transcripts in heterotopic subcutaneous tumors after injection of T24P (A) or HT-1376 cells (B) was determined by RT-PCR. "M": 100-bp molecular weight marker, lanes 1-2: heterotopic subcutaneous tumors from different mice induced by injection of T24P (A) or HT-1376(B) cells, lane 3: subcutaneous tissue of normal mouse, lanes 4: T24P (A) or HT-1376(B) cell lines, "C": negative control for PCR. The sizes of the PCR products are 300 bp for human H19, 119 bp for IGF2-P4 and 213 bp for Histone 3.3 internal control, respectively.