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Figure 10 | Journal of Translational Medicine

Figure 10

From: Development of targeted therapy for bladder cancer mediated by a double promoter plasmid expressing diphtheria toxin under the control of H19 and IGF2-P4 regulatory sequences

Figure 10

Detection of DTA and Luc transcripts in orthotopic bladder tumors: Mice with heterotopic bladder tumors were intravesically treated twice in 3 days interval, and were sacrificed 4 days after the last treatment. Tumors were excised and frozen immediately and 400 ng RNA (extracted from the tumors) was used for determination of luciferase and DTA by RT-PCR reaction. A). tumors treated with H19-Luc-P4-Luc (lanes 1-2), or with H19-DTA-P4-DTA (lanes 3-4). Lane 5: untreated orthotopic bladder tumor, 'C': negative control for PCR, 'M': 100 bp DNA ladder. The sizes of the PCR products are 468 bp and 328 bp, for DTA and Luc respectively. The lower panel shows the histone 3.3 internal control. Necrosis of orthotopic bladder tumor treated with H19-DTA-P4-DTA (H&E × 20) is shown (B) and the necrotic area is indicated (by green line). ('U', urothelium, 'LP', lamina propria, 'M', muscle).

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