Figure 5
From: Prevention of hyperglycemia-induced myocardial apoptosis by gene silencing of Toll-like receptor-4
Suppression of NADPH oxidase activity in TLR4-silenced STZ mice. Diabetes was induced by STZ injection as described in Materials and Methods. Diabetic mice were treated with TLR4 siRNA and scrambled control siRNA as described in Figure 2. On day 7 after STZ treatment, the hearts from mice treated with TLR4 siRNA (n = 6) or scrambled siRNA (n = 6) were retrieved, the protein was prepared and the NADPH oxidase activity was determined as described in Methods and Materials. Data are representative of 3 repeated experiments, and are shown as mean ± SD. (∗) Statistical significance when compared with scrambled siRNA treated mice was denoted as p < 0.05.