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Figure 7 | Journal of Translational Medicine

Figure 7

From: Thymoglobulin, interferon-γ and interleukin-2 efficiently expand cytokine-induced killer (CIK) cells in clinical-grade cultures

Figure 7

Generation of CIK cells with hiTG from patients with advanced solid cancer. The culture conditions described in Materials and Methods were used to generate CIK cells from the PBMC of 4 patients with advanced cancer. HiTG was used in these studies because it induced maximal expansion of CIK cells from healthy donor PBMC. Panel A: The absolute number of PBMC, CD3+CD8+ T cells, NK cells (CD3-CD16+CD56+) and CIK cells (CD3+CD16+CD56+) was estimated weekly after the provision of hiTG to the cultures. Results summarize (median and inter-quartile range) 4 independent experiments performed with PBMC preparations from 4 different patients. Panel B: The frequency of CD3+CD8+ T cells, NK cells (CD3-CD16+CD56+) and CIK cells (CD3+CD16+CD56+) from a representative PBMC sample is shown at baseline (day 0) and after 7, 14 and 21 days in culture. Quadrant markers were set according to the proper isotypic control (not shown). The percentage of cells staining positively for a given antigen is indicated. Panel C: Flow cytometry detection of intracellular FoxP3 in CD4+ T cells from a representative PBMC culture. Cells were fixed, permeabilized and labeled as detailed in Materials and Methods. The percentage of cells staining positively for intracellular FoxP3 is indicated both at baseline and after 21 days in culture. Quadrant markers were set according to the proper isotypic control (not shown). Panel D: The expression of NK-cell inhibitory/activating receptors was investigated by flow cytometry, as previously detailed. A representative experiment out of 4 with similar results is shown. Quadrant markers were set according to the proper isotypic control (not shown). The percentage of cells staining positively for a given antigen is indicated.

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