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Figure 4 | Journal of Translational Medicine

Figure 4

From: Thymoglobulin, interferon-γ and interleukin-2 efficiently expand cytokine-induced killer (CIK) cells in clinical-grade cultures

Figure 4

Frequency of bona fide Treg cells after the provision of either αCD3 mAb or TG to the cultures. Panel A: Cumulative frequency of CD4+FoxP3+ Treg cells within PBMC stimulated with either TG or αCD3 mAb. Data are expressed as median and inter-quartile range. Panels B and C: Flow cytometry detection of intracellular FoxP3 in CD4+ T cells at baseline (B) and after their in vitro expansion (C). Cells were fixed, permeabilized and labeled as detailed in Materials and Methods. Quadrant markers were set according to the proper isotypic control (not shown). The percentage of cells staining positively for intracellular FoxP3 is indicated.

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