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Table 2 Effect of NSC23766 and aspirin on P-selectin expression of PRP stimulated by ADP and collagen

From: Rac1-mediated signaling plays a central role in secretion-dependent platelet aggregation in human blood stimulated by atherosclerotic plaque

Agonist P-selectin expression
(% positive cells)
  PRP Aspirin-PRP
  Control Stimulated Control Stimulated
ADP (5 μM) 1.4 ± 0.7 6 ± 2.8 1 ± 0.5 5.4 ± 2.6
ADP+NSC23766 (300 μM) 1.2 ± 1 1.8 ± 1.3 0.9 ± 0.4 2.1 ± 1.5
Collagen (5 μg/ml) 3.3 ± 3.1 42.4 ± 16.9 2 ± 1.3 6 ± 3.6
Collagen+NSC23766 (300 μM) 1.8 ± 1.3 3.1 ± 2.7 2 ± 1.5 2 ± 1.8
  1. PRP or aspirin-pretreated PRP was incubated with NSC23766 (300 μM) or solvent (water) in the presence of 1 mM RGDS for 5 min whilst stirring at 37°C in the lumi-aggregometer before stimulation with ADP or collagen. P-selectin expression was measured by flow cytometry. Values are mean ± SD, n = 4.