Figure 2
Effect of NSC23766 on stimulus-induced ATP-secretion in blood. Blood was pre-incubated with or without 300 μM NSC23766 (for 5 min), with or without 2 mM RGDS (for 2 min; added 3 min after NSC23766 or H2O) whilst stirring at 37°C before stimulation with (A) TRAP (5 μM) and (B) collagen (0.5 μg/ml). Top, tracings of ATP-secretion of blood. Bottom, bar diagrams; numbers are % of maximal ATP-secretion induced by TRAP (5 μM) and collagen (0.5 μg/ml), respectively. Values are mean ± SD (n = 3-4). * p < 0.05.