GH1 therapy suppressed SREBP-1c activity and reduced the mRNA levels of SREBP-1-regulated genes encoding lipogenic enzymes in the livers of alcohol-fed mice. (A) Nuclear SREBP-1c protein levels. (B) Relative mRNA levels of hepatic SREBP-regulated lipogenic enzymes. A nonspecific nuclear protein band in nuclear extracts was used to confirm equal loading and to normalize the data. C: control group; GC: GH1-treated control group; A: alcohol group; GA: GH1-treated alcohol group; PI: pair-fed group I; PII: pair-fed group II. ACCα: acetyl-CoA carboxylase-α; FAS: fatty acid synthase; GPAT1: glycerol-3-phosphate acyltransferase; ME: malic enzyme; nSREBP-1: nuclear sterol regulatory element binding protein 1; SCD1: stearoyl coenzyme A desaturase 1; SREBP-1, sterol regulatory element binding protein 1. n = 6 mice per group. Means without a common letter differ at P < 0.05.