GH administration upregulated hepatic SIRT1 expression in alcohol-fed mice. (A) Hepatic mRNA expression of SIRT1. (B) SIRT1 protein levels. (C) Relative PGC1α acetylation. Hepatic nuclear SIRT1 protein levels were determined using an anti-SIRT1 antibody. A nonspecific nuclear protein band was used to confirm equal loading and to normalize the data. PGC1α was immunoprecipitated from liver extracts and immunoblotted with either an anti-acetylated lysine (Ac-Lys) antibody to determine the extent of PGC1α acetylation or with an anti-PGC1α antibody to determine the total amount of PGC1α. C: control group; GC: GH1-treated control group; A: alcohol group; GA: GH1-treated alcohol group; PI: pair-fed group I; PII: pair-fed group II. AOX: acyl-CoA oxidase; CPT1a: carnitine palmitoyltransferase 1a; MCDA: medium chain acyl-Co-A dehydrogenase; PGC1α: PPARα coactivator; PPARγ: peroxisome proliferator activated receptor-γ; SIRT1: sirtuin 1. n = 6 mice per group. Means without a common letter differ at P < 0.05.