Indirect immunofluorescence of mouse otospheres from second passage, cultivated in the presence of TGFα, and submitted to dish adherence and cell differentiation. Jagged 2 (panel A) and Myosin VIIa (panel B) are hair cell markers, here labeled in red and green, respectively. Arrows indicate their concentration near the plasma membrane, especially in membrane ruffles. P27kip1 labels supporting cell nuclei, as shown in panel C. DAPI identifies the cell nucleus in blue. Scale bar 10 μm.