Indirect immunofluorescence of mouse otospheres from second passage, cultivated in the presence of bFGF, and submitted to dish adherence and cell differentiation. Myosin VIIa, a marker for hair cells, is labeled by Alexa 488 and shown in panel A. Arrows indicate plasma membrane processes, underneath which there is an enrichment of myosinVIIa. P27kip1 and Jagged 1, markers for supporting cells give the expected green staining of plasma membrane and red labeling of nuclei, respectively, shown in panels B and C. DAPI stains in blue nuclear DNA. Scale bar 10 μm.