Detection of CD4+ and CD8+ T-cells and chemokines in regressing tumors of vaccinated mice. The MCA304 tumor samples in mice receiving PBS, the IL-13Rα2 DNA prime and ECDα2 boost vaccination were collected on days 33 from the experiment shown in Figure 3B and the immunohistochemistry and immunofluorescense microscopic analyses were done using antibodies specific for CD4 and CD8 (A and C) or CXCL9 and CXCL10 (B). IgG2 antibodies were used for isotype control (B). Three sections from each tumor samples were evaluated. The dark brown stained cells in Panel A indicate CD4+ and CD8+ T-cells. Magnification X20. Representative data from experiments done twice with a total 6 mice per group.