Prophylactic IL-13Rα2 DNA vaccination and post-tumor challenge boost with ECDα2. (A) prophylactic DNA vaccination of mice with MCA304, 4T1 and D5α2 tumors. Three IL-13Rα2 DNA vaccine or control vector (100 μg) were injected at two week interval before MCA304 (B and C), 4T1 (D and E), or D5α2 tumor (F and G) challenge in mice (n = 6). The ECDα2 boost vaccinations were injected on week 1 and 2. Tumor volumes were measured by Vernier caliper and Overall Sacrifice Time (OST) was calculated based on the sacrifice of mice when tumors reached to >2 cm. Experiment were repeated twice; bars, SD.