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Table 1 Primary antibodies used for immunohistochemical detection.

From: The tumor microenvironment of colorectal cancer: stromal TLR-4 expression as a potential prognostic marker

Primary antibody

Species raised in

Species directed to

Supplier (clone and/or #)

Dilution

CD68

Mouse

Human

DAKO (PG-M1, #M0876)

1: 100

CD15

Mouse

Human

DAKO (C3D-1, #M0733)

1: 20

CD31

Mouse

Human

DAKO (JC70A, #M0823)

1: 20

TLR-4

Rabbit

Human

SANTA CRUZ BIOTECHNOLOGY (M-300, #sc-30002) (Santa Cruz, California, USA)

1: 100

  

Mouse

NOVUS BIOLOGICALS (NB100-56581) (Littleton, CO, USA)

1:600

HGF

Goat

Human

SIGMA (H7157)

1: 400

IL-6

Rabbit

Human

NOVUS BIOLOGICALS

(NB600-1131)

1: 400

  

Mouse

ABCAM (ab6672) (Cambridge, UK)

1:400