Expression of TLR-4 in human colon tissues. 3a TLR-4 immunohistochemistry analysis. Different expression of TLR-4 in healthy tissues (A), ulcerative colitis (B), adenomas (C) and adenocarcinomas (D) show that increasing grade of dysplasia directly correlates with higher expression of this marker (magnification ×400). 3b Different expression of TLR-4 in endothelial, epithelial area and stromal department shows that in all groups TLR-4 is significantly increased respect to healthy tissues (mean ± SEM; **P < 0.01, *** P < 0.001). HT = healthy tissues (N = 16); UC = ulcerative colitis (N = 13), AD = adenomas (N = 34; 29 low and 5 high grade), AC = adenocarcinomas (N = 53; 7 T1, 10 T2, 33 T3, 3 T4).