Comparison of CD4 (A) and CD8 (B) growth when bead-treated T cells were restimulation with anti-CD3/CD28 beads (squares) or anti-CD3/irradiated MMCs (triangles). T cell expansion was monitored using CFSE labeled cells and flow cytometry as described in the methods. CD4 expansion was similar in response to either stimulus. By contrast, bead-treated CD8 cells expanded significantly less well than anti-CD3-treated cells at all time points. The p values with obtain using the Wilcoxon matched pairs test are indicated at the top of each box.