Figure 6

Early time course for expansion of bead-expanded T cells after restimulation 14 days later with anti-CD3/CD28 beads or anti-CD3 plus irradiated MMCs. Restimulated CD4 (A) and CD8 (C) both divided more extensively in response to anti-CD3 (dashed lines) than anti-CD3/CD 28 (solid lines), but this difference was more pronounced for CD8 cells. CD4 T cell expansion (C) was similar in response to either stimulus, but CD8 T cells (D) expanded substantially more rapidly than anti-CD3/CD28 bead-treated cells.